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Effect of Irradiation on the Incidence of Mating in Cactoblastis Cactorum (Scientific Notes) (Report)

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eBook details

  • Title: Effect of Irradiation on the Incidence of Mating in Cactoblastis Cactorum (Scientific Notes) (Report)
  • Author : Florida Entomologist
  • Release Date : January 01, 2009
  • Genre: Life Sciences,Books,Science & Nature,
  • Pages : * pages
  • Size : 65 KB

Description

In the Sterile Insect Technique (SIT) (Carpenter et al. 2001; Hight et al. 2005) used against the cactus moth, Cactoblastis cactorum Berg (Lepidoptera: Pyralidae), an invasive species threatening native Opuntia in the US Southwest and Mexico, large numbers of laboratory-reared adult moths are sterilized with ionizing radiation at 200 Gy (Tate et al. 2007) and released to mate with wild adults. The radiation dosage used is sufficient to render the females completely sterile but leave the males partially fertile. Progeny of irradiated males are few, predominantly male, and completely sterile (Carpenter et al. 2001; Tate et al. 2007). Success of the SIT depends on several variables, including the competitiveness of irradiated moths and the mating frequency of each gender. Our objectives were to determine whether irradiation compromised the mating ability of the males and whether adults would mate more than once. Two separate experiments were conducted to provide answers to these questions. In both experiments, adult males were irradiated with [sup.60]Co in a Gammacell 220 irradiator at a dose of 200 Gy 24 hr after eclosion. All insects were held in 400 cc ventilated cardboard cages at 25[degrees]C, 14:10 D:N, and 70-80% RH, with a small piece of cactus cladode and were unmated and 24 h old when placed on test.


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